Morphology and growth rates of HUVECs and ECFCs.

(A) A sample of HUVECs seeded on gelatin coverslips. After 72 hrs. of culture with a seeding density of 30,000 cells/cm2, cells were fixed and stained with ProLong Mountant with DAPI and anti-VWF FITC antibody to visualize nuclei and VWF, respectively. (B) The same was done for ECFCs, showing WPB distribution. (C) Nuclei count of HUVECs and ECFCs were evaluated at varying incubation time points (4, 24, 48, 72, 96 hrs.) with a constant seeding density of 30,000 cells/cm2 (n = 3). The dotted lines represent the growth rate curves as analyzed by a linear regression line of each cell line. The growth rate slopes for HUVECs and ECFCs are 0.39 and 0.30, respectively. The slopes are not significantly different, and P-value shown was determined by linear regression (GraphPad Prism).