Expression of gamma and alpha motor neuron markers in the absence of ERR2/3.

(A-H) Transversal sections of adult Chat::tdTomato lumbar mouse spinal cords of control (A-D) and ERR2/3cko (E-H) mice. (A-D) Expression of high levels of GFRA1 by ERR3high, NeuNlow, and tdTomato+ small soma-size motor neurons (arrowheads), consistently lower levels in larger some-size ERR3high, NeuNlow, and tdTomato+ motor neurons in control mice. (E-F) Persistent expression of high GFRA1 levels in NeuNlow small soma-size motor neurons (arrowheads) in ERR2/3cko mice. (I-T) Transversal sections of adult mouse spinal cords of control (I-K) and ERR2/3cko (L-N) mice. (I-K) High levels of expression of the fast-alpha motor neuron marker MMP9 by large some-size VACHT+ motor neurons in control mice. LMC, lateral motor column; MMC, medial motor column. (L-N) Persistent high MMP9 levels in large some-size VACHT+ motor neurons in ERR2/3cko mice. (O-T) Transversal sections of P400 mice spinal cords of control (O-Q) and ERR2/3cko (R-T) mice. (O-Q) High levels of expression of the slow/intermediate alpha motor neuron marker OPN by subsets of intermediate some-size VACHT+ motor neurons in control mice. (R-T) Persistent high OPN levels in subsets of large some-size VACHT+ motor neurons in ERR2/3cko mice. (Scale bars: 100 μm). (TIF)