Phylogenetic analyses of flaviviruses.

Phylogenetic trees were constructed based on the RdRp sequence of representative viruses in the family Flaviviridae (A), the segment 3 of ALSV and other representative viral strains in the Jingmenvirus group (B), the E protein of TBEV in NE China (C), and the RdRp of BLTV4 (D). All the viruses obtained in ticks here were highlighted in red. In panel A, closest referenced viruses were highlighted in bold font, and the virus genera or groups were marked with different colors of background. In panel B, ALSV isolated from humans were marked with blue. ALSV were highlighted with light green background. The host tick species of the viruses and the countries that the viruses discovered were also labeled. In panel C, TBEV isolated from CBM and XXAM were marked with blue-filled circles, while strains found in DXAM were marked with red-filled circles. In panel D, the strain names of BLTV4 were labeled. The host tick species and the countries that BLTV4 discovered were also marked. ALSV, Alongshan virus; TBEV, Tick-borne encephalitis virus; BLTV4, Bole tick virus 4. The accession numbers of the viral sequences are shown in S2 and S3 Tables.