In-Silico Approaches of Polyphenols and In-Vivo Evaluation of Neuroprotective Effects of Eugenia Jambolana Leaves Extract for Anticholinesterase and Antioxidant Activities

Eugenia jambolana Lam. (Myrtaceae) is widely used in India to treat several ailments in the traditional system of medicine. The present study focused on some use of plant Eujenia jambolna which is experimentally proven with some studies like HPTLC, and animal model. The insilico study was performed to study the neuroprotective effect and the mechanism of these plants, phytoconstituents by using proteins AChE and BuChE, for Methyl gallate, Myricetrin, and Nilocitin and Rivastigmine is used as standard. The central cholinergic system plays an important role in symptoms and sighs of alzheimers. In the present study, Eujenia jambolna leaves extracts was administered orally in two doses 200mg and 400 mg/kg to assess the learning and memory. AlCl₃ was used to induce AD in rat and the study is performed in model Morris water maze. The study proves that the extract has a neuroprotective action proved by decreasing escape latency morris water model, and has a AChE inhibition action which is proved by Ellman’s test. Furthur more studies with isolated bioactives from Eugenia Jambolana and making novel drug delivery formulation for Alzheimers. The HPTLC study report confirmed that it contains 7 to 15 phytoconstituents and by using standard Ascorbic Acid the antioxidant activity of the extract was performed by analyzing the % inhibition by analyzing the area inhibited in DPPH scavenging activity which showed neuroprotective action of the extract. Furthur studies can bring out plant derived drugs with less side effects when compared to existing allopathic drugs. Further studies in future can be carried out to elucidate the other neurotransmitter to evaluate the mechanism of action, clinical studies may for carried out to establish its efficacy in humans