15-2-14 :細胞壁合成･修飾･分解機構の研究

Modification of peptidoglycan is important in various bacteria. Especially Bacillus subtilis produces spore under the starvation conditions. We investigated the modification of spore peptidoglycan called cortex with the use of the polysaccharide deacetylase gene (pdaA) of Bacillus subtilis. PdaA-deficient spore did not lead to germination and this mutant completely lacked muramic-delta-lactam which is essential to germination. In-vitro experiments indicated that PdaA did not catalyze deacetylation reaction when the native polysaccharide was used as a substrate, but catalyzed deacetylation when the amidase-digested polysaccharide was used as a substrate. These results indicate PdaA plays a key role in cortex biosynthesis. On the other hand, cell surface targeting of peptidoglycan hydrolases was investigated by the immuno-fluorescence microscopy with 3xFLAG tagged protein, and localization of three proteins was identified.Article先進ファイバー工学研究教育拠点研究成果報告書 10: 60-60(2004)research repor