15-2-8 :キチン･キトサンの酵素変換による高機能化に関する研究

A gene (csnC) coding for an extracellular chitosaanse was isolated from the koji mold, Aspergillus oryzae, and a complete nucleotide sequence was determined. Chitosanase cDNA derived from the fungus Fusarium solani was expressed in Saccharomyces cerevisiae. By using this expression system, two amino acid residues responsible for catalysis were determined, and an inverting-type reaction mechanism was also confirmed. A group of genes specifically expressed at the stage of fruit body development was isolated from the basidiomycete Flammulina velutipes. One of the genes encoded a polypeptide having a primary sequence similar to those of polysaccharide deacetylases. A metagenome Library was constructed from choromosomal DNAs prepared from whole bacterial cells in soil. From this library, E. coli transformant exhibiting a red fluorescent light was isolated and a gene structure in the cloned DNA fragment was analyzed.Article先進ファイバー工学研究教育拠点研究成果報告書 10: 52-53(2004)research repor