Change of Serum Protein of Laying Hen under Various Feeding Conditions and Egg-Laying

This experiment was performed by liquid chromatography and starch gel electrophoresis in order to study alterations in the serum protein of the same hen under different feeding conditions; semi-purified diets (20% and 10% protein diet and protein free diet) and fasting. Moreover, change of serum protein of a hen being in good egg production at different times before oviposition was observed. Albumin in the blood serum remarkably increased just before and after oviposition. It was suggested that this may have something to concern with the formation of egg white in the oviduct. The fraction appearing at pH 4.0 may be related to egg shell membrane formation and egg white secretion. And, there was minor effect of protein source in the diet on this fraction. The twelve fractions were found in the electrograph under the feeding of formula diet. Fractions 6 and 8 disappeared as fed with 20% protein diet. The picture of electrophoresis on the feeding of protein-free diet was similar to that of formula ration. But, it was observed that the fractions below 9 in position for the protein-free diet considerably decreased in quantity in comparison with the fractions for both formula ration and 20% protein diets. Judging from the position in the picture, perhaps these fractions seemed to contain r-globulin. These were perceptively decreased by the feeding of protein-free diet and fasting. As a whole, it was noticed that albumin and globulin fractions appear to vary with the above feeding conditions inversely. On the electrophoretical analysis of egg albumen, lysozyme fraction was found to be most in the inner thin albumen.Article信州大学農学部紀要 6(1): 47-54(1969)departmental bulletin pape