Revalorization of chicken feather waste into a high antioxidant activity feather protein hydrolysate using a novel psychrotolerant bacterium
- Bezus, Brenda
- Ruscasso, Maria Florencia
- Garmendia, Gabriela
- Vero, Silvana
- Cavello, Ivana Alejandra
- Cavalitto, Sebastian Fernando
DOIAbstract
In this study, we assessed the capacity of Pedobacter sp. 3.14.7 —a novel Antarctic keratinolytic bacterium— of producing a feather protein hydrolysate (FPH) with high antioxidant activity. This strain was able to degrade raw feather into protein hydrolysate under optimal conditions of 30 g l−1 of feather concentration, pH 6.0, and 20 °C. Maximum enzymatic activity was detected after a 192-h cultivation period (125.67 ± 3.06 U ml−1). Quenching of the ABTS radical (2,20-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid) by the hydrolysate reached a value of approximately 95%, with a 50% Inhibitory Concentration (IC50) value of 0.57 ± 0.01 g l−1. An interesting reducing power of the FPH towards Fe+3 was also detected (2.19 Abs700 units in th...
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