Development and characterization of 21 microsatellite markers for Pancratium maritimum L. (Amaryllidaceae)

Pancratium maritimum L. (Amaryllidaceae) is a bulbous perennial plant growing on coastal sands of Mediterranean, Black and Caspian seas as well as on part of European coasts of Atlantic Ocean. The excess of flowers sampling, urbanization and tourism put serious threats to the species, resulting in a significant decrease of its populations. In order to investigate its conservation genetics, we developed and characterized microsatellite markers in P. maritimum. Twenty-one microsatellite loci were isolated using the SSRpatchwork protocol. The average number of alleles per locus was 4.4. Polymorphic information content value ranged from 0 to 0.75 with an average of 0.39. Eleven loci showed significant departures from Hardy–Weinberg equilibrium