Identification of an active dimeric intermediate populated during the unfolding process of the cambialistic superoxide dismutase from Streptococcus mutans

  • MERLINO, ANTONELLO
  • RUSSO KRAUSS, IRENE
  • VERGARA, ALESSANDRO
  • MARCO, SALVATORE
  • DE VENDITTIS, EMMANUELE
  • SICA, FILOMENA
  • Rossi B.
  • De Vendittis A.
Publication date
January 2012
ISSN
0300-9084
Citation count (estimate)
4

Abstract

Superoxide dismutases are enzymes that protect biological systems against oxidative damage caused by superoxide radicals. In this paper, a detailed characterization is presented on the stability of SmSOD, the dimeric cambialistic superoxide dismutase from the dental pathogenic microorganism Streptococcus mutans, towards temperature and guanidine hydrochloride. Thermal and chemical denaturations were investigated by means of circular dichroism, fourth-derivative UV spectroscopy and fluorescence measurements. Data indicate that SmSOD is endowed with a significant thermostability and that both its thermal and guanidine hydrochloride-induced unfolding processes occur through a three-state model, characterized by a catalytically active dimeric i...

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