The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rather stable transcript encompassing the five distal cistrons. The molecular mechanisms by which such a transcript is produced were investigated in Escherichia coli strains carrying mutations in several genes for exo- and endonucleases. The experimental approach made use of S1 nuclease protection assays on in vivo synthesized transcripts, site-directed mutagenesis and construction of chimeric plasmids, dissection of the processing reaction by RNA mobility retardation experiments, and in vitro RNA degradation assays with cellular extracts. We have found that processing requires (1) a functional endonuclease E; (2) target site(s) for this activit...
Escherichia coli RNase E is known to process RNA precursors at specific sites. We show that this end...
The best characterized pathway for the initiation of mRNA degradation in Escherichia coli involves t...
Escherichia coli endoribonuclease E has a major influence on gene expression. It is essential for th...
The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rat...
The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rat...
Endonuclease processing of mRNA derived from the rif region near 90 minutes on the Escherichia coli ...
Gene 32 from bacteriophage T4 is transcribed as precursor transcripts which are processed to a stabl...
We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium....
We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium....
The hypothesis generally proposed to explain the stabil-izing effect of translation on many bacteria...
The endonuclease RNase E of Escherichia coli is essential for viability, but deletion of its C-termi...
In Escherichia coli, 5'-terminal RNA stem-loop structures are major impediments to the rate-determi...
In order to adapt to changing environmental conditions and regulate intracellular events such as div...
Ribonuclease P (RNase P) is an endoribonuclease required for the maturation of the 5 ' termini ...
Recently E.coli endoribonuclease RNase E has been implicated in the functional and chemical decay of...
Escherichia coli RNase E is known to process RNA precursors at specific sites. We show that this end...
The best characterized pathway for the initiation of mRNA degradation in Escherichia coli involves t...
Escherichia coli endoribonuclease E has a major influence on gene expression. It is essential for th...
The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rat...
The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rat...
Endonuclease processing of mRNA derived from the rif region near 90 minutes on the Escherichia coli ...
Gene 32 from bacteriophage T4 is transcribed as precursor transcripts which are processed to a stabl...
We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium....
We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium....
The hypothesis generally proposed to explain the stabil-izing effect of translation on many bacteria...
The endonuclease RNase E of Escherichia coli is essential for viability, but deletion of its C-termi...
In Escherichia coli, 5'-terminal RNA stem-loop structures are major impediments to the rate-determi...
In order to adapt to changing environmental conditions and regulate intracellular events such as div...
Ribonuclease P (RNase P) is an endoribonuclease required for the maturation of the 5 ' termini ...
Recently E.coli endoribonuclease RNase E has been implicated in the functional and chemical decay of...
Escherichia coli RNase E is known to process RNA precursors at specific sites. We show that this end...
The best characterized pathway for the initiation of mRNA degradation in Escherichia coli involves t...
Escherichia coli endoribonuclease E has a major influence on gene expression. It is essential for th...