Schematic presentation of the CRISPR/Cas9-Knock out screen.

Julian Schoellkopf (14259688)
Thomas Mueller (33067)
Lena Hippchen (14259691)
Teresa Mueller (14259694)
Raphael Reuten (2583358)
Rolf Backofen (11285)
Joachim Orth (14259697)
Gudula Schmidt (413226)

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Publication date

December 2022

DOI

10.1371/journal.ppat.1010781.g001

Publisher

Public Library of Science (PLoS)

Abstract

Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral CRISPR-library. Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.</p

Extracted data

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Schematic presentation of the CRISPR/Cas9-Knock out screen.

Abstract

Extracted data

Schematic presentation of the CRISPR/Cas9-Knock out screen.

Abstract

Extracted data

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