3D rotational diffusion of micrometric wires using 2D video microscopy

We propose a simple way to measure the three-dimensional rotational diffusion of micrometric wires, using two-dimensional video microscopy. The out-of-plane Brownian motion of the wires in a viscous fluid is deduced from their projection on the focal plane of an optical microscope objective. An angular variable reflecting the out-of-plane motion, and satisfying a Langevin equation, is computed from the apparent wire length and its projected angular displacement. The rotational diffusion coefficient of wires between 1 and 100 mu m is extracted, as well as the diameter distribution. Translational and rotational diffusion were found to be in good agreement. This is a promising way to characterize soft visco-elastic materials, and probe the dimension of anisotropic objects. Copyright (C) EPLA, 201