Survey of the presence of β-agonist agents contain in meat by immunity rapid test method and LC-MS/MS in Nantou County

[[abstract]]本論文之目的在於完整調查南投市內乙型接受體致效劑(β-agonist agents)瘦肉精含量狀況，並建立乙型接受體致效劑(β-agonist agents)瘦肉精萊克多巴胺(Ractopamine)在肉品中以免疫層析技術之分析技術，檢驗樣品包括生鮮冷藏肉品及冷凍肉品，另為釐清是否有含有瘦肉精的台灣生產豬肉存在，故進一步以 21 種瘦肉精進行調查，委託中央畜產會檢驗機構，以衛授食字第1101901019號 食品中動物用藥殘留量檢驗方法－乙型接受體致效劑類多重殘留分析，110年5月1日至110年5月20日隨機收集南投市售 42 件牛肉類及豬肉類為檢體，結果可為台灣養殖業肉瘦精使用現況，作一完整調查，並可完成污染背景值分佈，並進行討論風險評估及研訂限量標準。乙型接受體致效劑 (β-agonist agents)，原用於人類或豬馬牛等畜牧業氣管疾病之用藥，後來發現豬馬牛等畜牧肉類使用瘦肉精後可增加畜牧肉類的瘦肉比例，減少養殖時間，獲得較大的生產效益，但人類若食用殘留瘦肉精的肉品後，會有疑慮有非選擇性或選擇性乙型接受體致效劑的藥理機轉，輕者心悸或肌肉震顫或暈眩嘔吐，孕婦小孩或相關疾病者食用後更會有嚴重後果，為了消弭民眾食的疑慮，本研究採用的方法為商用萊克多巴胺商用套組，最低靈敏度2 ppb，使用簡單方便，整個操作步驟過程 5 分鐘可完成。本論文依牛豬加強抽驗計畫，針對知名餐飲業、火鍋店、攤販販售業者等風險較高業者，原預計抽驗50件產品，實際執行抽驗共42件，執行期間於110年5月1日起展開肉品瘦肉精體檢，截至5月20日止共抽驗42件檢體，其中牛肉類18件（42％）、豬肉類24件（58％），以萊克多巴胺商用套組驗出 1 件美國牛肉樣品含萊克多巴胺。另為釐清是否有含有瘦肉精的台灣生產豬肉存在，針對21種乙型接受體致效劑(β-agonist agents)進行分析檢驗，送驗42件產品結果共有34件（80％）未檢出，4件（10％）檢出，4件（10％）無報告，檢出之檢體種類均為牛肉，檢出之瘦肉精為 ractopamine，檢出數值之範圍為0.002至0.007 ppm，檢出國家均為美國。依據行政院衛福部公告之「動物用藥殘留標準」，台灣生產肉品中皆不得檢出乙型接受體致效劑，台灣生產豬肉均未檢出21種瘦肉精，可保障民眾食在安心的權益。商用萊克多巴胺商用套組與液相層析串聯質譜儀法比對，其中1件與公告方法結果相符，但有3件出現偽陰性，建議檢驗應以政府正式公告方法之結果為判斷依據。[[abstract]]The purpose of this study was to investigate the presence of β-agonist agents contain in meat in Nantou County. We first established the Elisa immunity rapid test method of analysis of β-agonist agent, then the samples of meat were collected from traditional markets or retail shops and analyzed for the β-agonist agents. The kit uses the principle of immunochromatography assay. It can detect ractopamine in muscle samples. After adding the sample solution into the sample well of detection card, ractopamine of the sample solution combine with the gold-labelled antibody, so as to prevent the combining of gold-labelled antibody with RAC conjugate on the cellulose membrane.The Elisa ractopamine rapid test kit method was applied to 42 samples of meat, β-agonist agent was found in one sample and detected in more than the 0.02 ppb. The results showed that β-agonist agents in in Nantou County were low risk in meat, it has to been continuing monitor to protect consumer health. The LC-mass-mass method was applied to 42 samples of meat. Ractopamine was detected in two samples measurable at 0.002-0.007 ppm. In conclusion, this study had shown that the Elisa rapid test kit and HPLC methods could be applied successfully to analyze β-agonist agents occurred in meat in Nantou County