Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophoretic protein pattern. This invalidates the electrophoretic evaluation of the content in the secretion from the accessory sex glands. Both agarose and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the proteolytic changes and how they could be modified by various synthetic protease inhibitors. Liquefaction of coagulated semen could be inhibited by adding o-phenanthroline directly after ejaculation, whereas neither neutrally buffered Na2EDTA, di-isopropylfluorophosphate (DFP), benzamidine, nor thiol reagents proved effective. Addition of the serine protease inhibitors DFP and benzamidine, o-phenanthroline...
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigat...
Human seminal plasma proteins from fertile and infertile men were fractionated by electrophoresis. T...
Summary. The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic ins...
Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophore...
Human seminal plasma contains a protein factor that can inhibit the motility of both demembranated-r...
The predominant protein in human seminal vesicle secretion constitutes the structural protein of coa...
Liquefaction of coagulated human semen was inhibited by o-phenanthroline; subsequent addition of Zn2...
This study was conducted to evaluate changes in ram seminal plasma composition from ejaculates obtai...
ABSTRACT: Secretory leucocyte protease inhibitor, SLPI, is a low‐molecular‐weight, acid‐stable prote...
most concentrated in the initial portion of the ejaculate that consists mostly ofCowper's gland...
Seminal plasma is semen without the spermatozoa. Human seminal plasma is a complex mixture of secret...
Human seminal plasma is known to possess considerable proteolytic activity, much of which is associa...
In addition to kallikrein hK3, a serine protease generafly reported as PSA (prostate-specific antige...
Freshly ejaculated sperm acquire the fertilizing potential by a continuing process that occurs durin...
Proteomics is a research area that has developed rapidly in the last decade. It studies the large-sc...
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigat...
Human seminal plasma proteins from fertile and infertile men were fractionated by electrophoresis. T...
Summary. The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic ins...
Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophore...
Human seminal plasma contains a protein factor that can inhibit the motility of both demembranated-r...
The predominant protein in human seminal vesicle secretion constitutes the structural protein of coa...
Liquefaction of coagulated human semen was inhibited by o-phenanthroline; subsequent addition of Zn2...
This study was conducted to evaluate changes in ram seminal plasma composition from ejaculates obtai...
ABSTRACT: Secretory leucocyte protease inhibitor, SLPI, is a low‐molecular‐weight, acid‐stable prote...
most concentrated in the initial portion of the ejaculate that consists mostly ofCowper's gland...
Seminal plasma is semen without the spermatozoa. Human seminal plasma is a complex mixture of secret...
Human seminal plasma is known to possess considerable proteolytic activity, much of which is associa...
In addition to kallikrein hK3, a serine protease generafly reported as PSA (prostate-specific antige...
Freshly ejaculated sperm acquire the fertilizing potential by a continuing process that occurs durin...
Proteomics is a research area that has developed rapidly in the last decade. It studies the large-sc...
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigat...
Human seminal plasma proteins from fertile and infertile men were fractionated by electrophoresis. T...
Summary. The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic ins...