Infrared Thermal Imaging and Morpho-Physiological Indices Used for Wheat Genotypes Screening under Drought and Heat Stress

Bread wheat, one of the largest broadacre crops, often experiences various environmental stresses during critical growth stages. Terminal drought and heat stress are the primary causes of wheat yield reduction worldwide. This study aimed to determine the drought and heat stress tolerance level of a group of 46 diverse wheat genotypes procured from the Australian Grains Gene Bank, Horsham, VIC Australia. Two separate drought stress (DS) and heat stress (HS) pot experiments were conducted in separate growth chambers. Ten days after complete anthesis, drought (40 ± 3% field capacity for 14 days) and heat stress (36/22 °C for three consecutive days) were induced. A significant genotype × environment interaction was observed and explained by various morpho-physiological traits, including rapid, non-destructive infrared thermal imaging for computational water stress indices. Except for a spike length in DS and harvest index in HS, the analysis of variance showed significant differences for all the recorded traits. Results showed grains per spike, grains weight per spike, spike fertility, delayed flag leaf senescence, and cooler canopy temperature were positively associated with grain yield under DS and HS. The flag leaf senescence and chlorophyll fluorescence were used to measure each genotype’s stay-green phenotype and photosystem II activity after DS and HS. This study identified the top ten best and five lowest-performing genotypes from drought and heat stress experiments based on their overall performance. Results suggest that if heat or drought adaptive traits are brought together in a single genotype, grain yield can be improved further, particularly in a rainfed cropping environment