Search for host factors located in the vicinity of the cytoplasmic tails of the MuV F and HN proteins.

(A) Schematic diagrams of F-FLAG-Tb(C) and Tb(N)-HA-HN. (B) Schematic overview of the split-TurboID-based proximity biotinylation using a virus-like particle (VLP) assay system. (C) VLP assay showing the amounts of the N and M proteins expressed in the cells (Lysate) and released into the supernatants (Sup). (D) Immunoprecipitation assay showing the interaction of F-FLAG-Tb(C) protein with Tb(N)-HA-HN and M proteins in 293T cells. (E) Immunoblotting showing the ligase activity of split-TurboID inserted into the cytoplasmic domains of MuV F and HN proteins in 293T cells. (F) Immunoblotting showing the biotinylated F-FLAG-Tb(C) and Tb(N)-HA-HN proteins in 293T cells. (G) Immunofluorescence assay showing the biotinylated proteins in Vero cells expressing F-FLAG-Tb(C) and Tb(N)-HA-HN proteins. The experiments were performed at least three times, and representative data are shown.