Cell behavior categories and their distributions.

For each category, a representative time series for a single cell (top, with blue representing nuclear membrane permeability, green representing calcium concentration, and red representing mitochondrial polarity) along with a heat map of cell counts indicating relative frequency at different locations are shown. Colorbar maxima vary between categories to resolve spatial distributions for categories with fewer cells. Regions 1–3 are on the impacted sample, with 1 as the site of impact. Regions 4–5 are on the control sample. a) The nuclear membrane permeability starts out elevated and decays. b) The nuclear membrane permeability increases within 30 min of impact and plateaus or continues increasing. c) The nuclear membrane permeability increases within 30 min of impact and then decays. d) The nuclear membrane permeability increases after 30 min after impact but no prior signaling event is observed. e) The nuclear membrane permeability increases despite the mitochondria being polarized. f) The nuclear membrane permeability increases after the calcium concentration drops after a period of sustained elevation. g) Transient calcium signaling is observed after the nuclear membrane permeability has already increased. h) The nuclear membrane permeability increases after calcium transient(s). i) Calcium transient(s) are observed with no increase in nuclear membrane permeability. j) No calcium transients or increases in nuclear membrane permeability. k) All three signals are very low. l) The nuclear membrane permeability increases multiple times.