Boxplots of mean FA and volumes of ROIs.

Boxplot comparing mean FA values (A) and volume in mm3 (B) in n = 15 healthy controls for 4 segmentation methods of the body, genu and splenium of the corpus callosum (BCC, GCC, SCC respectively), fornix and cingulum bilaterally. The four segmentation methods include SSDS, a manual segmentation on the parametric map, crossing ROI with normalized FA in MNI152 template space, and extracting the mean skeletonised FA value of each ROI mask. Although the fornix was omitted from analysis (because of a poor test -retest reliability), we included segmentation of the fornix to illustrate comparisons of the methods on a small tract. Ns = not significant, *p <0.05, **p <0.01, ***p <0.001.