No involvement of CFTR negative regulators in CFTR inhibition by DHLV.

(A) Schematic diagrams showing the regulatory mechanism of CFTR Cl- channel activity. (B) apical Cl- current (ICl-) tracing showing the effect of α,β-dehydrolovastatin (DHLV) on forskolin-induced CFTR Cl- secretion in T84 cells pre-treatment with compound C (50 μM) or and NaF plus Na3VO4 (1 mM) for 15 min. (C) Summary of concentration-inhibition studies. Data are fitted to Hill’s equation and expressed as means of % agonist-stimulated ICl- ± S.E.M. (n = 5–9).