LIMK1 and Cofilin regulate activation of JNK in <i>hid-p35</i> clones.

(A-F’) Late 3rd instar wing discs with 48-hour old mosaic clones positively marked by GFP. MMP1 (magenta in A-F and grey in A’-F’) is a marker of JNK activity. Images of Z-stack projections are shown as the MMP1 signals localize at the basal part of the disc. A low level of MMP1 signals was detected in the control clones expressing P35 (A, A’). In contrast, a strong increase of MMP1 labelling was observed in the clones simultaneously expressing hid and p35 (hid-p35 clones) (B, B’). Such a strong level of MMP1 signals is largely inhibited by expression of droncRNAi (C, C’), bskDN (D, D’), or in LIMK12 mutants (E, E’). Expression of cofilin also suppresses the hid-p35-induced MMP1 expression (F, F’). (G) Quantification of the MMP1 signal intensity in the representative wing disc pouches carrying clones of the indicated genotypes. Compared to the discs with p35 clones, the MMP1 signals are significantly (****p hid-p35 clones. This increase is significantly (****p droncRNAi, bskDN, cofilin or in Tak1 hemizygous null mutants or LIMK12 mutants.