Pull-down assay indicated the presence of interaction between the MiaB and Vfr proteins.
- Qiqi Lin (7140776)
- Jiahui Huang (794375)
- Zhiqing Liu (1664941)
- Qunyi Chen (14216420)
- Xinbo Wang (1430794)
- Guohui Yu (538898)
- Ping Cheng (289811)
- Lian-Hui Zhang (119133)
- Zeling Xu (4104238)
Publication date
December 2022
DOI Publisher
Public Library of Science (PLoS)Abstract
Pull-down assay was performed using glutathione beads with whole cell lysate of E. coli BL21 which expresses the GST-tagged MiaB protein (GST-MiaB) or/and whole cell lysate of BL21(DE3) which expresses the His-tagged Vfr protein (His-Vfr). GST-tagged MiaB was analyzed by immunoblotting with anti-GST antibody (upper panel) while the His-tagged Vfr protein was analyzed by immunoblotting with anti-His antibody (lower panel). (TIF)</p
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