Feedback

The T3SS operon <i>exsCEBA</i> in <i>P</i>. <i>aeruginosa</i> was positively regulated by an adenosine tRNA methylthiotransferase PA3980 (MiaB).

  • Qiqi Lin (7140776)
  • Jiahui Huang (794375)
  • Zhiqing Liu (1664941)
  • Qunyi Chen (14216420)
  • Xinbo Wang (1430794)
  • Guohui Yu (538898)
  • Ping Cheng (289811)
  • Lian-Hui Zhang (119133)
  • Zeling Xu (4104238)
Open link
Publication date
December 2022
Icon DOI
10.1371/journal.ppat.1011027.g001
Publisher
Public Library of Science (PLoS)

Abstract

(A) β-galactosidase activity of the PexsCEBA-lacZ transcriptional fusion in the wide-type PAO1 strain, ΔPA3980, and ΔPA3980 with in trans expression of PA3980. (B) β-galactosidase activity of the PexsCEBA-lacZ transcriptional fusion in the wide-type PAO1 strain, ΔPA3980, and ΔPA3980 with in trans expression of miaB from E. coli (miaB-E). (C) LC-MS measurement of the tRNA A37 N6-isopentenyladenosine (i6A) and 2-methylthio-N6-isopentenyladenosine (ms2i6A) in the wide-type PAO1 strain and ΔPA3980 mutant. ns, not significant; ***, P t test.</p

Extracted data

We use cookies to provide a better user experience.
Data Protection