<i>gacA</i>, <i>rsmY</i>, <i>rsmZ</i> were repressed by MiaB to activate T3SS gene expression.

(A) Relative expression of gacA, rsmY and rsmZ measured by RT-qPCR in the strains of wide-type PAO1, ΔmiaB, and ΔmiaB with in trans expression of miaB. (B) Relative expression of gacA measured by RT-qPCR in the strains of wide-type PAO1, ΔmiaB, ΔladS and ΔmiaBΔladS. (C) Relative expression of rsmY and rsmZ measured by RT-qPCR in the strains of wide-type PAO1, ΔmiaB, ΔgacA and ΔmiaBΔgacA. (D) Production of the ExoS protein measured by western blot in the wide-type PAO1 strain, ΔmiaB, ΔgacA, ΔrsmY, ΔrsmZ and double or triple deletion mutants. ns, not significant; **, P P t test.