Polymorphism of the apolipoprotein E gene in the population of the indigenous inhabitants of the Mountain Shoria and its association with the parameters of carbohydrate metabolism

Objective: we investigated apolipoprotein E gene polymorphism and its influence on a carbohydrate metabolism in native population of Mountain Shoria (West Siberia). Methods: The study included 40 men and 80 women aged 25-64. The apolipoprotein E polymorphism was analyzed by original method using Hixson’s approach. The serum glucose levels were determined by standard enzymatic assays. Results: The frequencies of ε2, ε3, ε4 alleles in men were 6.5 %, 80.4 %, 13.1 % and in women – 7.6 %, 72.6 %, 19.8 % respectively. The frequencies of genotypes ε2/ε4, ε2/ε3, ε3/ε3, ε3/ε4, ε4/ε4 in men and women together were 1.8 %, 12.4 %, 51.8 %, 31.2 % and 2.9 %. Mean fasting serum glucose levels in case of genotypes ε2/ε4, ε2/ε3, ε3/ε3, ε3/ε4 and ε4/ε4 were 4.6±0.3mg/dl, 4.6±0.1mg/dl, 4.0±0.1mg/dl, 4.7±0.1mg/dl, 5.8±0.3mg/dl (рGLM=0,000) in man and 4.7±0.3mg/dl, 4.7±0.1mg/dl, 4.1±0.1mg/dl, 4.9±0.1mg/dl, 5.9±0.2mg/dl in women (рGLM=0,000). After glucose tolerance test mean serum glucose levels in case of genotypes ε2/ε4, ε2/ε3, ε3/ε3, ε3/ε4 and ε4/ε4 were 6.0±0.6mg/dl, 6.1±0.3mg/dl, 5.2±0.2mg/dl, 6.4±0.2mg/dl, 8.4±0.5mg/dl (рGLM=0,000) in man and 6.2±0.6mg/dl, 6.1±0.2mg/dl, 5.5±0.1mg/dl, 6.7±0.1mg/dl, 8.6±0.5mg/dl (рGLM=0,000) in women. Conclusions: The native population of Mountain Shoria is characterized by relatively high frequencies of ε4 alleles. The allele ε4 prevails in women. The genotype ε4/ε4 has been associated with higher serum glucose level