NF-KB involvement for CTLA4-IG intracellular signaling in human macrophages

Background Transcription factor NF-kB (nuclear factor kB) play an important role in the regulation of immune and inflammatory responses [1]. Previous studies showed that a significant downregulation of pro-inflammatory cytokine expression (TNF\u3b1, IL-1\u3b2 and IL-6) was evident for cultured human macrophages treated with CTLA4-Ig fusion protein [2]. Objectives The aim of the study was to investigate the NF-kB pathway as possible intracellular signaling target involved in the CTLA4-Ig modulation of cytokine production (TNF\u3b1, IL-1\u3b2 and IL-6) in cultured human macrophages. Methods THP1 cell line, differentiated by PMA (0.5 \u3bcg/ml for 3 hours) into adherent macrophages, were seeded in tissue culture dishes in culture medium with CTLA4-Ig [100 and 500 \u3bcg/ml for 3 and 12 hours] or without CTLA4-Ig, for mRNA extraction and qRT-PCR analysis. At the end of incubation adherent cells were harvested and mRNA was extracted and processed by qRT-PCR analysis for NF-kB1 (p50 subunit) and for TNF\u3b1, IL-1\u3b2, IL-6. Experiments were done in triplicate. Results The qRT-PCR analysis of NFKB1 mRNA, after 3 and 12 hours from CTLA4-Ig treatment, showed a significant downregulation (p<0.001) of the gene expression vs. cnt at both concentrations [100 and 500 \u3bcg/ml]. At the same time, the qRT-PCR analysis of inflammatory cytokine mRNA, already after 3 hours from treatment, showed that CTLA4-Ig [100 \u3bcg/ml] induced a significant decrease for TNF\u3b1 and IL-6 gene expression (p<0.05), compared to untreated macrophages (cnt). CTLA4-Ig [500 \u3bcg/ml] was able to reduce TNF\u3b1 gene expression vs. cnt in a larger extent (p<0.001). After 12 hours from treatment with CTLA4-Ig [100, 500 \u3bcg/ml], it was still evident a significant downregulation in cytokine gene expression for IL-1\u3b2 and IL-6 vs. cnt (p<0.001). Interestingly, TNF\u3b1 downregulation was not still significant after 12 hours from treatment. Conclusions The action of CTLA4-Ig may be exerted at level of the intracellular signaling (trascription factors) by downregulating NF-kB1 gene expression, together with a significant reduction in gene expression for tested inflammatory cytokines