Live-cell 3D super-resolution imaging in thick biological samples
- Francesca Cella Zanacchi
- Michela Perrone Donnorso
- Alessio Del Bue
- Laura Furia
- Mario Faretta
- LAVAGNINO, ZENO
- DIASPRO, ALBERTO GIOVANNI
Publication date
January 2011
DOI Publisher
Nature Publishing Group ISSN
1548-7091 Citation count (estimate)
232Abstract
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids
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