A preliminary dose-dependent test for Fig 1C based on the readouts of ten indicated AR mutants (WT as control) in response to steroidal ligands. The OD600 values of liquid yeast cultures (without the presence of 3-AT) were measured 21 h post-incubation. Bars indicate mean ± s.d. (n = 2). (TIF)</p
<p>The indicated yeast strains were arrested in G1 with mating pheromone and divided into two sample...
Recently we constructed recombinant yeast cells that express the human androgen receptor (hAR) and y...
In vitro assays are considered as the first step in a tiered approach to compound screening for horm...
(A) Liquid dose-dependent yeast assay with 25 mM 3-AT added (n = 5). OD600 values were measured 48 h...
The “+” mark indicates a full response of the AR mutants to indicated ligands was observed in plate ...
(A) Schematic representation of our designer yeast platform compared with the mammalian luciferase r...
(A) Characterization of the AR-LBD EP-PCR library. (B) Yeast clonal selection against compounds base...
T878A was a E2/PROG/CPA-responsive AR mutant (n = 4 in liquid yeast assays). (TIF)</p
Cancer progression is associated with the evolutionary accumulation of genetic mutations that are bi...
(A) Serial cell dilutions of the indicated yeast stains were cultivated on media without any drugs (...
<p>A. Complementation assay of the yeast <i>tps1Δ tps2Δ</i> mutant. B. Complementation assay of the ...
Values are normalized to that for Pgk1p, used as an internal control and related to the values for W...
<p>Different concentrations of kaempferol (µM) were added to genetically engineered, estrogen- respo...
Cancer progression is associated with the evolutionary accumulation of genetic mutations that are bi...
<p>A. Alignment of 2 tryptic phospho-peptides across 6 yeast species. Possible phosphorylation sites...
<p>The indicated yeast strains were arrested in G1 with mating pheromone and divided into two sample...
Recently we constructed recombinant yeast cells that express the human androgen receptor (hAR) and y...
In vitro assays are considered as the first step in a tiered approach to compound screening for horm...
(A) Liquid dose-dependent yeast assay with 25 mM 3-AT added (n = 5). OD600 values were measured 48 h...
The “+” mark indicates a full response of the AR mutants to indicated ligands was observed in plate ...
(A) Schematic representation of our designer yeast platform compared with the mammalian luciferase r...
(A) Characterization of the AR-LBD EP-PCR library. (B) Yeast clonal selection against compounds base...
T878A was a E2/PROG/CPA-responsive AR mutant (n = 4 in liquid yeast assays). (TIF)</p
Cancer progression is associated with the evolutionary accumulation of genetic mutations that are bi...
(A) Serial cell dilutions of the indicated yeast stains were cultivated on media without any drugs (...
<p>A. Complementation assay of the yeast <i>tps1Δ tps2Δ</i> mutant. B. Complementation assay of the ...
Values are normalized to that for Pgk1p, used as an internal control and related to the values for W...
<p>Different concentrations of kaempferol (µM) were added to genetically engineered, estrogen- respo...
Cancer progression is associated with the evolutionary accumulation of genetic mutations that are bi...
<p>A. Alignment of 2 tryptic phospho-peptides across 6 yeast species. Possible phosphorylation sites...
<p>The indicated yeast strains were arrested in G1 with mating pheromone and divided into two sample...
Recently we constructed recombinant yeast cells that express the human androgen receptor (hAR) and y...
In vitro assays are considered as the first step in a tiered approach to compound screening for horm...