Ovarian follicular dynamic and LH profile in heifers treated in the Experiment 2.

A) Growth profile of dominant follicle in pubertal heifers treated at Day 6 of follicular wave (day of wave emergence = Day 0) with human Kisspeptin-10 given as three injections of 15 mg at 60 minutes interval over a 2-hour period (Kp10 group, n = 5). Cetrorelix group (n = 5) was injected GnRH antagonist (Cetrorelix, 20 μg/Kg body weight, intramuscular) 3 hours before kisspeptin treatment while the Control group (n = 5) was given three intravenous injections of normal saline. B) Plasma LH concentrations in Kp10, Cetrorelix and Control group heifers.