CHO/LY-B Cell Growth under Limiting Sphingolipid Supply: Correlation between Lipid Composition and Biophysical Properties of Sphingolipid-Restricted Cell Membranes

Sphingolipids (SL) are ubiquitous in mammalian cell membranes, yet there is little data on the behavior of cells under SL- restriction conditions. LY- B cells derive from a CHO linein whichserine palmitoyl transferase (SPT), thus de novo SL synthesis, is suppressed, while maintaining the capacity of taking up and metabolizing exogenous sphingoid bases from the culture medium. In this study, LY- B cells were adapted to grow in a fetal bovine serum (FBS)- deficient medium to avoid external uptake of li-pids. The lowest FBS concentration that allowed LY- B cell growth, though at a slow rate, under our conditions was 0.04%, that is, 250- fold less than the standard (10%) concentration. Cells grown under limiting SL concentrations remained viable for at least 72hours. Enriching with sphingomyelin the SL- deficient medium allowed the recovery of growth rates analogous to those of control LY- B cells. Studies in-cluding whole cells, plasma membrane preparations, and derived lipid vesicles were carried out. Laurdan fluorescence was recorded to measure membrane molecular order, showing a significant decrease in the rigidity of LY- B cells, not only in plasma membrane but also in whole cell lipid extract, as a result of SL limitation in the growth medium. Plasma membrane preparations and whole cell lipid extracts were also studied using atomic force microscopy in the force spectroscopy mode. Force measurements demonstrated that lower breakthrough forces were required to pen-etrate samples obtained from SL- poor LY- B cells than those obtained from control cells. Mass- spectroscopic analysis was also a helpful tool to understand the rear-rangement undergone by the LY- B cell lipid metabolism. The most abundant SL in LY- B cells, sphingomyelin, decreased by about 85% as a result of SL limitation in the medium, the bioactive lipid ceramide and the ganglioside precursor hexosylcera-mide decreased similarly, together with cholesterol. Quantitative SL analysis showed that a 250- fold reduction in sphingolipid supply to LY- B cells led only to a sixfold decrease in membrane sphingolipids, underlining the resistance to changes in com-position of these cells. Plasma membrane compositions exhibited similar changes, at least qualitatively, as the whole cells with SL restriction. A linear correlation was observed between the sphingomyelin concentration in the membranes, the degree of lipid order as measured by laurdan fluorescence, and membrane breakthrough forces assessed by atomic force microscopy. Smaller, though significant, changes were also detected in glycerophospholipids under SL- restriction conditions.This work was supported in part by grants from the Spanish Ministry of Economy (grant FEDER MINECO PGC2018-099857-B-I00) and the Basque Government (grants No. IT1264-19 and IT1270-19), as well as Fundación Biofísica Bizkaia and the Basque Excellence Research Centre (BERC) program of the Basque Government, and by the Swiss National Science Foundation (310030-184949