Park9 interaction with Manganese and other divalent cations

Two peptide sequences from Park9 Parkinson’s disease gene, P1D2E3K4H5E6L7 (1) and F1C2G3D4G5A6N7D8C9G10 (2) have been studied in their interaction with Mn(II) and Zn(II) ions. These fragments lie from residue 1165 to 1171 and from 1184 to 1193 in the Park9 encoded protein, that can protect cells from manganese poisoning, an environmental risk factor for a Parkinson’s disease-like syndrome called Manganism. The study was carried out through potentiometric and spectroscopic (UV-Vis, EPR, mono- and multidimensional NMR) techniques, to cast light on the details of metal binding at different pH values and different ligand to metal molar ratios