BIO GRIP AND MACHINED TITANIUM STIMULATE DENTAL PULP STEM CELLS TOWARDS OSTEOBLASTIC DIFFERENTIATION

Biomaterials have been utilized routinely during maxillofacial, craniofacial, and orthopaedic reconstructive surgical procedures. To investigate which one between BIO GRIP and machined titanium disk promote a greater osteoblast differentiation and proliferation, the expression levels of bone related genes (RUNX2, SP7, ALPL, SPP1, COL1A1, COL3A1 and FOSL1) and mesenchymal stem cells marker (ENG) were measured in dental pulp stem cells (DPSCs) after 15 and 30 days of treatment using real time Reverse Transcription-Polymerase Chain Reaction. Significantly differentially expressed genes among Bio Grip treated and untreated cells were ENG, FOSL1, RUNX2, COL3A1, BGLAP and SPP1 in the first 15 days of treatment and ENG, FOSL1, RUNX2,COL3A1 and BGLAP after 30 days. Conversely, all genes were differentially expressed among treated and untreated Machined Titanium DPSCs after 15 days. At the end of the exposure, SP7, COL3A1, COL1A1 and ALPL were the only gene differentially expressed. The present study demonstrated both biomaterials, are able to induce bone formation by influencing the expression pattern of gene involved in osteogenesis, extracellular matrix deposition and mineralizatio