Surface plasmon resonance based biosensor technology for real-time detection of PCR products

In this chapter, we reviewed different experimental approaches for PCR-based biospecific interaction analysis (BIA) employing surface plasmon resonance (SPR) and biosensor technologies: choice of primers and probes, characterization of PCR-products immobilized on flow cells, use of PNA as molecular probes, injection of asymmetric PCR products to flow cells carrying specific probes. We discussed of the advantages of SPR-based BIA for characterization of PCR products, problems and possible solutions and future perspectives