Macrophages loaded with doxorubicin by ATP-mediated permeabilization: potential carriers for antitumor therapy
- M. MUNERATI
- R. CORTESI
- D. FERRARI
- F.DI VIRGILIO
- C. NASTRUZZI
DOIAbstract
In a number of cell types extracellular ATP (ATPe) is known to cause reversible plasma membrane permeabilization to low molecular weight water soluble compounds (Mr<900 D). In the present report we have exploited this technique to charge with the anticancer drug doxorubicin the cytoplasm of J774 and L929 cells. These two mouse cell lines were previously characterized for the presence of the ATPe-gated pore, J774 macrophages and tumor necrosis factor (TNF)-resistant L929 fibroblasts. ATPe-mediated reversible permeabilization allowed an at least a fourfold increase of doxorubicin intracellular trapping with respect to passively loaded cells (2 pg/cell versus 0.5 pg/cell respectively). The analysis of the doxorubicin release kinetic at 37°C fr...
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