Supplementary material 5 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Figure 2 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109

Anslan, Sten
Nilsson, R. Henrik
Wurzbacher, Christian
Baldrian, Petr
Tedersoo, Leho
Bahram, Mohammad

September 2018

Figure 2 - OTU accumulation curves of the evaluated pipelines for a) PacBio and b) Illu...

Supplementary material 1 from: Shumskaya M, Lorusso N, Patel U, Leigh M, Somervuo P, Schigel D (2023) MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris. MycoKeys 96: 77-95. https://doi.org/10.3897/mycokeys.96.101033

Shumskaya, Maria
Lorusso, Nicholas
Patel, Urvi
Leigh, Madison
Somervuo, Panu
Schigel, Dmitry

March 2023

Sequences of the primers used in the experiment and ITS2 fragment amplified from DNA extracted from ...

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.

Michiel Op De Beeck
Bart Lievens
Pieter Busschaert
Stéphan Declerck
Jaco Vangronsveld
Jan V Colpaert

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...

Supplementary material 4 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Table S4. Data set of the ITS1 barcode.: Explanation note: Data set of the ITS1 barcode

Supplementary material 6 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Table S6. Data set of the LSU D1, D2, and D3 barcodes.: Explanation note: Data set of the LSU D1, D2...

Figure 2 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 2 - Sample-based OTU richness as recovered by different barcode-primer pair combinations. Err...

Figure 7 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 7 - Differences in sequence length in the ITS1 and ITS2 barcodes of 16 most abundant fungal c...

Figure 1 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 1 - Map of ribosomal DNA indicating variable regions as well as primers used and/or discussed...

Figure 4 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 4 - Relationship between connectance and adjusted coefficient of determination (R2adj) for fl...

Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Rapid development of high-throughput (HTS) molecular identification methods has revolutionized our k...

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies

Op De Beeck, Michiel
Lievens, Bart
Busschaert, Pieter
Declerck, Stéphan
Vangronsveld, Jaco
Colpaert, Jan V

June 2014

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...

Supplementary material 3 from: Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. MycoKeys 88: 1-33. https://doi.org/10.3897/mycokeys.88.77106

Ceballos-Escalera, Angelina
Richards, John
Arias, Maria Belen
Inward, Daegan J. G.
Vogler, Alfried P.

March 2022

Figure S3. Maximum-likelihood tree constructed in IQ-Tree2 based on three-gene (LSU D1-D2, SSU, ITS2...

Filtered sequencing data (MOTUs) for fungal metabarcode

Pansu, Johan
Winkworth, Richard C.
Hennion, Françoise
Gielly, Ludovic
Taberlet, Pierre
Choler, Philippe

July 2015

This table contains filtered MOTUs for the fungal metabarcode. Amplicons were amplified using ITS5 :...

Unfiltered sequencing data for fungal metabarcode (table format)

July 2015

This table contains pre-filtered sequencing data (i.e. usable merged reads assigned to their origina...

Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies

Michiel Op De Beeck (578438)
Bart Lievens (147571)
Pieter Busschaert (578439)
Stéphan Declerck (578440)
Jaco Vangronsveld (198587)
Jan V. Colpaert (578441)

June 2014

<div><p>Current metabarcoding studies aiming to characterize microbial communities generally rely on...

Figure 2 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109

Anslan, Sten
Nilsson, R. Henrik
Wurzbacher, Christian
Baldrian, Petr
Tedersoo, Leho
Bahram, Mohammad

September 2018

Figure 2 - OTU accumulation curves of the evaluated pipelines for a) PacBio and b) Illu...

Supplementary material 1 from: Shumskaya M, Lorusso N, Patel U, Leigh M, Somervuo P, Schigel D (2023) MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris. MycoKeys 96: 77-95. https://doi.org/10.3897/mycokeys.96.101033

Shumskaya, Maria
Lorusso, Nicholas
Patel, Urvi
Leigh, Madison
Somervuo, Panu
Schigel, Dmitry

March 2023

Sequences of the primers used in the experiment and ITS2 fragment amplified from DNA extracted from ...

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.

Michiel Op De Beeck
Bart Lievens
Pieter Busschaert
Stéphan Declerck
Jaco Vangronsveld
Jan V Colpaert

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...

Supplementary material 4 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Table S4. Data set of the ITS1 barcode.: Explanation note: Data set of the ITS1 barcode

Supplementary material 6 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Table S6. Data set of the LSU D1, D2, and D3 barcodes.: Explanation note: Data set of the LSU D1, D2...

Figure 2 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 2 - Sample-based OTU richness as recovered by different barcode-primer pair combinations. Err...

Figure 7 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 7 - Differences in sequence length in the ITS1 and ITS2 barcodes of 16 most abundant fungal c...

Figure 1 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 1 - Map of ribosomal DNA indicating variable regions as well as primers used and/or discussed...

Figure 4 from: Tedersoo L, Anslan S, Bahram M, Põlme S, Riit T, Liiv I, Kõljalg U, Kisand V, Nilsson RH, Hildebrand F, Bork P, Abarenkov K (2015) Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1-43. https://doi.org/10.3897/mycokeys.10.4852

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Figure 4 - Relationship between connectance and adjusted coefficient of determination (R2adj) for fl...

Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

Tedersoo, Leho
Anslan, Sten
Bahram, Mohammad
Põlme, Sergei
Riit, Taavi
Liiv, Ingrid
Kõljalg, Urmas
Kisand, Veljo
Nilsson, Henrik
Hildebrand, Falk
Bork, Peer
Abarenkov, Kessy

May 2015

Rapid development of high-throughput (HTS) molecular identification methods has revolutionized our k...

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies

Op De Beeck, Michiel
Lievens, Bart
Busschaert, Pieter
Declerck, Stéphan
Vangronsveld, Jaco
Colpaert, Jan V

June 2014

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...

Supplementary material 3 from: Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. MycoKeys 88: 1-33. https://doi.org/10.3897/mycokeys.88.77106

Ceballos-Escalera, Angelina
Richards, John
Arias, Maria Belen
Inward, Daegan J. G.
Vogler, Alfried P.

March 2022

Figure S3. Maximum-likelihood tree constructed in IQ-Tree2 based on three-gene (LSU D1-D2, SSU, ITS2...

Filtered sequencing data (MOTUs) for fungal metabarcode

Pansu, Johan
Winkworth, Richard C.
Hennion, Françoise
Gielly, Ludovic
Taberlet, Pierre
Choler, Philippe

July 2015

This table contains filtered MOTUs for the fungal metabarcode. Amplicons were amplified using ITS5 :...

Unfiltered sequencing data for fungal metabarcode (table format)

July 2015

This table contains pre-filtered sequencing data (i.e. usable merged reads assigned to their origina...

Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies

Michiel Op De Beeck (578438)
Bart Lievens (147571)
Pieter Busschaert (578439)
Stéphan Declerck (578440)
Jaco Vangronsveld (198587)
Jan V. Colpaert (578441)

June 2014

<div><p>Current metabarcoding studies aiming to characterize microbial communities generally rely on...

Figure 2 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109

Anslan, Sten
Nilsson, R. Henrik
Wurzbacher, Christian
Baldrian, Petr
Tedersoo, Leho
Bahram, Mohammad

September 2018

Figure 2 - OTU accumulation curves of the evaluated pipelines for a) PacBio and b) Illu...

Supplementary material 1 from: Shumskaya M, Lorusso N, Patel U, Leigh M, Somervuo P, Schigel D (2023) MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris. MycoKeys 96: 77-95. https://doi.org/10.3897/mycokeys.96.101033

Shumskaya, Maria
Lorusso, Nicholas
Patel, Urvi
Leigh, Madison
Somervuo, Panu
Schigel, Dmitry

March 2023

Sequences of the primers used in the experiment and ITS2 fragment amplified from DNA extracted from ...

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.

Michiel Op De Beeck
Bart Lievens
Pieter Busschaert
Stéphan Declerck
Jaco Vangronsveld
Jan V Colpaert

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...

Abstract

Extracted data

Abstract

Extracted data

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