The present work shows that salt anions affect the activity of Pseudomonas cepacia lipase both in aqueous and in nonaqueous media (NAM) according to a Hofmeister series. The biocatalytic assay in water was the hydrolysis of p-nitrophenyl acetate, whereas the esterification between 1-hexyl-beta-D-galactopyranoside and palmitic acid was followed in an organic solvent. The solid lipase preparations to be used in NAM were obtained through lyophilization in the presence of concentrated solutions of Hofmeister salts (Na2SO4, NaH2PO4/Na2HPO4, NaCl, NaBr, NaI, NaSCN). Salts affect enzyme activity in organic media through two mechanisms: (1) enzyme protection during lyophilization; (2) enzyme activation during the reaction. At least in our case, the...
The discovery that enzymes can function in organic solvents has broadened the scope of biocatalysis,...
The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia l...
The catalytic activities of lyophilized powders of α-chymotrypsin and Candida antarctica lipase were...
The present work shows that salt anions affect the activity of Pseudomonas cepacia lipase both in aq...
The present work shows that salt anions affect the activity of Pseudomonas cepacia lipase both in aq...
The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity ...
The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity ...
The specific activity of lipase A (Aspergillus niger) toward the hydrolysis of p-nitrophenyl acetate...
Pseudomonas fluorescens lipase (Pfl) was adsorbed on macroporous polypropylene to obtain a heterogen...
The effects of weak and strong electrolytes on the enzymatic activity of Candida rugosa lipase are e...
The specific activity of lipase A (Aspergillus niger) toward the hydrolysis of p-nitrophenyl acetate...
Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was stud...
Lipases from oilseeds have a great potential for commercial exploration as industrial enzymes. Lipas...
Most of lipases are in equilibrium between a majority inactive closed form and a minority active ope...
ABSTRACT: Extracellular lipase was isolated and purified from culture broth of bacillus species. Th...
The discovery that enzymes can function in organic solvents has broadened the scope of biocatalysis,...
The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia l...
The catalytic activities of lyophilized powders of α-chymotrypsin and Candida antarctica lipase were...
The present work shows that salt anions affect the activity of Pseudomonas cepacia lipase both in aq...
The present work shows that salt anions affect the activity of Pseudomonas cepacia lipase both in aq...
The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity ...
The effects of a kosmotropic phosphate buffer (PB) or sodium sulfate (SS) on the enzymatic activity ...
The specific activity of lipase A (Aspergillus niger) toward the hydrolysis of p-nitrophenyl acetate...
Pseudomonas fluorescens lipase (Pfl) was adsorbed on macroporous polypropylene to obtain a heterogen...
The effects of weak and strong electrolytes on the enzymatic activity of Candida rugosa lipase are e...
The specific activity of lipase A (Aspergillus niger) toward the hydrolysis of p-nitrophenyl acetate...
Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was stud...
Lipases from oilseeds have a great potential for commercial exploration as industrial enzymes. Lipas...
Most of lipases are in equilibrium between a majority inactive closed form and a minority active ope...
ABSTRACT: Extracellular lipase was isolated and purified from culture broth of bacillus species. Th...
The discovery that enzymes can function in organic solvents has broadened the scope of biocatalysis,...
The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia l...
The catalytic activities of lyophilized powders of α-chymotrypsin and Candida antarctica lipase were...