Gram-negative bacteria are protected by an outer membrane barrier, and to reach their periplasmic target, penicillins have to diffuse through outer membrane porins such as OmpF. Here we propose a structure-dynamics-based strategy for improving such antibiotic uptake. Using a variety of experiments (high-resolution single channel recording, Minimum Inhibitory Concentration (MIC), liposome swelling assay) and accelerated molecular simulations, we decipher the subtle balance of interactions governing ampicillin diffusion through the porin OmpF. This suggests mutagenesis of a hot spot residue of OmpF for which additional simulations reveal drastic changes in the molecular and energetic pathway of ampicillin's diffusion. Inverting the problem, w...
Gram-negative bacteria are protected against external attack by an outer membrane. However, general...
ABSTRACT To study translocation of b-lactam antibiotics of different size and charge across the oute...
In Gram-negative bacteria membrane-protein channels, also known as porins, constitute the entry poin...
In Gram-negative bacteria, the outer membrane porin F (OmpF) constitute the preferred entry point of...
Introduction: The increasing bacterial resistance to antibiotics poses significant threat in treatme...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
In Gram-negative bacteria, the outer membrane porin F (OmpF) constitute the preferred entry point of...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
Introduction: The increasing bacterial resistance to antibiotics poses significant threat in treatme...
Our aim in this study was to provide an atomic description of ampicillin translocation through OmpF,...
AbstractOmpF from the outer membrane of Escherichia coli is a general porin considered to be the mai...
Our aim in this study was to provide an atomic description of ampicillin translocation through OmpF,...
SummaryThe structure of OmpF porin in complex with three common antibiotics (zwitterionic ampicillin...
Gram-negative bacteria are protected against external attack by an outer membrane. However, general...
Gram-negative bacteria are protected against external attack by an outer membrane. However, general...
ABSTRACT To study translocation of b-lactam antibiotics of different size and charge across the oute...
In Gram-negative bacteria membrane-protein channels, also known as porins, constitute the entry poin...
In Gram-negative bacteria, the outer membrane porin F (OmpF) constitute the preferred entry point of...
Introduction: The increasing bacterial resistance to antibiotics poses significant threat in treatme...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
In Gram-negative bacteria, the outer membrane porin F (OmpF) constitute the preferred entry point of...
OmpF from the outer membrane of Escherichia coli is a general porin considered to be the main pathwa...
Introduction: The increasing bacterial resistance to antibiotics poses significant threat in treatme...
Our aim in this study was to provide an atomic description of ampicillin translocation through OmpF,...
AbstractOmpF from the outer membrane of Escherichia coli is a general porin considered to be the mai...
Our aim in this study was to provide an atomic description of ampicillin translocation through OmpF,...
SummaryThe structure of OmpF porin in complex with three common antibiotics (zwitterionic ampicillin...
Gram-negative bacteria are protected against external attack by an outer membrane. However, general...
Gram-negative bacteria are protected against external attack by an outer membrane. However, general...
ABSTRACT To study translocation of b-lactam antibiotics of different size and charge across the oute...
In Gram-negative bacteria membrane-protein channels, also known as porins, constitute the entry poin...