Enantiomeric Separation of Amino-alcohols on Dinitrobenzoyl-diaminocyclohexane Chiral Stationary Phases

A silica-bonded chiral stationary phase, containing the N,N'-3,5-dinitrobenzoyl derivative of 1R,2R-diaminocyclohexane, was used to separate the enantiomers of some amino alcohols with beta-blocking activity after their conversion to oxazolidin-2-ones. The influence of mobile phase composition (mixtures of hexane with dichloromethane, chloroform, dioxane and isopropanol) on the enantioselectivity and efficiency of the column was evaluated. Furthermore, a tandem arrangement of the chiral column and its racemic version was used to resolve all the stereoisomers of one amino alcohol containing two stereogenic centres