Table_1_Dynamic changes in fractional amplitude of low-frequency fluctuations in patients with chronic insomnia.DOC

BackgroundPrevious neuroimaging studies have mostly focused on changes in static functional connectivity in patients with chronic insomnia (CI). Features of dynamic brain activity in patients with CI have rarely been described in detail. The present study investigated changes in dynamic intrinsic brain activity in patients with CI by dynamic fractional amplitude of low-frequency fluctuation (dfALFF) analysis.Materials and methodsA total of 30 patients with CI and 27 healthy controls (HCs) were enrolled. We compared dfALFF between these two groups, and examined the correlation between changes in dfALFF and clinical symptoms of CI. Multivariate pattern analysis was performed to differentiate patients with CI from HCs.ResultsCompared with HC subjects, patients with CI showed significantly increased dfALFF in the left insula, right superior temporal gyrus, left parahippocampal gyrus, right amygdala, and bilateral posterior lobes of the cerebellum. Moreover, dfALFF values in the left insula and left parahippocampal gyrus showed a positive correlation with Pittsburgh Sleep Quality Index scores. A logistic regression model was constructed that had 96.7% sensitivity, 80.0% specificity, and 83.0% overall accuracy for distinguishing patients with CI from HCs.ConclusionDynamic local brain activity showed increased instability in patients with CI. The variability in dfALFF in the limbic system and brain areas related to sleep/wakefulness was associated with insomnia symptoms. These findings may provide insight into the neuropathologic basis of CI.