Enzymatic synthesis of EPA- and DHA-enriched structured acylglycerols at the sn-2 position starting from commercial salmon oil and concentrated by response surface methodology under supercritical conditions

Poster.-- 50th Conference of the Western European Fish Technologists Association, Rotterdam, Netherlands, 17th - 21st October 2022Marine oils are widely used due to their high content of omega 3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) since its consumption is associated with a low prevalence of heart, circulatory and inflammatory diseases. The bio-availability of such fatty acids has been shown to depend on their location in triacylglycerol (TG) mulecules at the sn-2 position. Consequently, great attention has been accorded to the synthesis of structured acylglycerols (SAcyl), which includes EPA or DHA at the sn-2 position. Supercritical fluids, such as carbon dioxide (C01), have solvent power. lts density can be changed and manipulated as a function of pressure and temperature since, at its critical pressure, its compressibility is maximum and small changes in thermal parameters can lead to significant changes in local density. Different types of biocatalysts, such as lipases, can be used to modify lipids. They are the most versatile since their regiospecificity is one of the main advantages for modifying oils and fats to obtain high added-value products, such as EPA + UHA supplements. This work aimed to optimize the synthesis of a SAcyl from commercial refined salmon oil (CRSO) and a concentrate of n-3 LCPUFA obtained from the same oil to enhance the positioning of EPA and DHA in sn-2 in glycerol backbone by enzymatic acidolysls in supercritical C02 (C02SC)ANID/CONICYT + FONDECYT Regular + folio (1181774 y 1221633).N