Bioevaluation of synthetic pyridones as dual inhibitors of alpha-amylase and alpha-glucosidase enzymes and potential antioxidants

Herein, a library of novel pyridone derivatives 1-34 was designed, synthesized, and evaluated for alpha-amylase and alpha-glucosidase inhibitory as well as antioxidant activities. Pyridone derivatives 1-34 were synthesized via a one-pot multi-component reaction of variously substituted aromatic aldehydes, acetophenone, ethyl cyanoacetate, and ammonium acetate in absolute ethanol. Synthetic compounds 1-34 were structurally characterized by different spectroscopic techniques. Most of the tested compounds showed more promising inhibition potential than the standard acarbose (IC50 = 14.87 +/- 0.16 mu M) but compounds 13 and 12 were found to be the most potent compounds with IC50 values of 9.20 +/- 0.14 mu M and 3.05 +/- 0.18 mu M against alpha-amylase and alpha-glucosidase enzymes, respectively. Compounds 1-34 also displayed moderate antioxidant potential in the range of IC50 = 96.50 +/- 0.45 to 189.98 +/- 1.00 mu M in comparison to the control butylated hydroxytoluene (BHT) (IC50 = 66.50 +/- 0.36 mu M), in DPPH radical scavenging activities. Additionally, all synthetic derivatives were subjected to a molecular docking study to investigate the interaction details of compounds 1-34 (ligands) with the active site of enzymes (receptors). These results indicate that the newly synthesized pyridone class may serve as promising lead candidates for controlling diabetes mellitus and as antioxidants.Higher Education Commission of Pakistan SHEC/SRSP/Med-3/15/2021-