Genetic Assessment of Silver Carp Populations in River Chenab (Pakistan) as Revealed by SSR Markers

Freshwater fish stocks are being exposed to increasing threats as a result of fisheries and aquaculture practices. Integrating genetic knowledge into fisheries and aquaculture management is becoming increasingly important in order to ensure the sustainability of species. So, I used SSR markers to evaluate the pattern of genetic variability in Silver Carp populations (175 samples) from five different sites of River Chenab, Pakistan. DNA was isolated and processed for analysis. There were no scoring errors related to large allele, no stuttering bands, and no null allele. The mean values of number of alleles, allelic richness, effective number of alleles, observed (Ho) and expected (He) heterozygosites, 1-Ho/He, inbreeding coefficient, pairwise population differentiation, and the gene flow provided data indicating loss of genetic diversity of silver carp in River Chenab (Pakistan). Reasons are overhunting, pollution, inbreeding, and poor control measures