Effect of Platelet Additive Solution (PAS) on Random Donor Platelet Concentrates

Background: To observe in vitro parameters ofstored platelets in PAS as compared to plasma for 10days.Methods: A comparative study was carried out on42 samples of random donor platelet concentrates(PC). They were divided into two groups of 21samples each and stored in incubator at 22°C. In onegroup plasma was replaced with PAS. Sampling wasdone on day 0, 3, 5, 7, 8, 9 and 10. Bacterial cultureswere carried out. All the samples,which showedcontamination, were excluded from the study.Results: pH and glucose levels were reduced inboth groups but they dropped more rapidly in PCstored in plasma than in PAS (p-value ≤ 0.001).However, the difference in LDH levels between twogroups was not statistically significant. The meanvalues of PO2 were reduced whereas mean values ofPCO2 were increased in both groups, but thesechanges were seen much less in PC stored in PAS (pvalue≤ 0.001). The results showed that difference inall parameters between two groups was highlysignificant (p-value ≤ 0.001) except LDH.Conclusion: All the in vitro parameters were wellpreserved in PC stored in PAS than in plasma