Effect of Caffeine and Vitamin D3 on Body Weight of Balb/C Mice

Background: To evaluate effect of caffeine andvitamin D3 on body weight of BALB/c mice.Methods: In this experimental study thirty BALB/cmice, three weeks old with average weight 12-14 gwere taken and divided into three groups with 10mice (5 male, 5 female) in each group. The controlgroup G1 was given normal diet prepared at NationalInstitute of Health Islamabad with liberal waterintake. Each animal in experimental group G2 wasgiven 10mg of caffeine per100g body weight onalternate day, three days in a week by oral gavage for60 days. All animals of G3 were given caffeine10mg/100g body weight on alternate day, three daysa week and vitamin D3 0.1μg per day by oral gavagefor 60 days. The effect of caffeine and vitamin D3was assessed by measuring the body weight of themice at the end of the experiment.Results: Mean final body weight of mice of controlgroup G1 was 31.2 ± 1.932 g while the mean finalbody weight of experimental groups G2 and G3 wasfound to be 27.1 ± 2.025 g and 29.2 ± 2.098 g . Meanfinal body weight of mice of experimental group G2was less as compared to control group G1 andexperimental group G3.Conclusion: The body weight of the mice wasreduced due to caffeine consumption while vitaminD3 administration ameliorated the deleterious effectof caffeine on the body weight