Evidence of the non ribosomal biosynthetic mechanism in the formation of syringomycin and syringopeptin, bioative lipodepsipeptides of the phytopathogenic bacterium Pseudomonas syringae pv. syringae

Syringomycin and syringopeptins are lipodepsipeptide metabolites produced by the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Radioactively labelled lipopeptides were produced in whole cell cultures supplemented with radioactively labelled putative biosynthetic precursors in the presence of the protein synthesis inhibitor, chloramphenicol. The results indicate that these lipopeptides are formed by a nonribosomal mechanism of peptide synthesis