Additional file 2 of Lactobacillus reuteri tryptophan metabolism promotes host susceptibility to CNS autoimmunity

Additional file 2: Figure S2. Expression levels of putative aromatic amino acid aminotransferase (ArAT) loci identified in the L. reuteri genome. (A) Pathway schematic of bacterial and abbreviated mammalian tryptophan metabolism from Fig. 2A. Enzymes with genomic evidence in Lactobacillus isolates are boxed in orange (ArAT), blue (FldH) and yellow (AmiE). (B) Heatmap of bacterial tryptophan specific enzymes with genomic evidence in Lactobacillus isolates. Enzymes are listed along the left in corresponding colors to the pathway in (A) with isolates and representative strains of the same species along the top and warmer colors indicating increasing copy number. (C) Expression level of araT loci in L. reuteri following 4 or 24hrs (D) of monoculture with 0 or 1mM tryptophan supplementation in brain heart infusion (BHI) medium as measured by qRT-PCR. Data is organized top to bottom corresponding to the heatmap in (B). Cultures were performed in triplicate and expression levels are normalized to a pan-Eubacterial primer set against the 16S rRNA gene. Primer sets are available in Table S35