Additional file 5 of Lactobacillus reuteri tryptophan metabolism promotes host susceptibility to CNS autoimmunity

Additional file 5: Figure S5. Abundance dynamics of L. reuteri in experimental breeding pairs and within dietary intervention studies. Founder G0 B6-GF mice were inoculated with cryopreserved donor cecal contents from PWD, B6, or B6 cecal contents supplemented with 109 CFU of L. reuteri and breeding pairs were established for vertical transmission to G1 offspring. Fecal samples were collected at 4-wks post-inoculation and in experimental offspring prior to dietary intervention (pre-diet), following 1-week of randomized diets (naive) and following a full 30-day disease course (post-EAE). Abundance of L. reuteri and L. murinus was determined by qPCR using species specific primers in (A) G0 breeders and G1 offspring and (B) throughout the course of dietary intervention. Relative abundance of L. reuteri at day 30 post EAE induction was correlated with disease severity as measured by cumulative disease score (CDS) using linear regression with the P value indicating significant deviation from a non-zero slop (i.e. significant correlation) for pre-diet (C), naive (D), and post-EAE (E) samples