Additional file 9 of Lactobacillus reuteri tryptophan metabolism promotes host susceptibility to CNS autoimmunity

Additional file 9: Figure S9. L. reuteri metabolites are sufficient to elicit a Th17 immune response. Splenocytes were differentiated under Th17 conditions without or without IGoxA, IAA, and/or AhR antagonist treatment at 0, 1, 10 or 100 μM followed by intracellular cytokine staining and flow cytometry. CD4+ and CD8+ T cell (A) frequency of total CD45+ T cells and IL-17 production (B and C) as frequency of each parent population in response to IGoxA treatment with and without AhR inhibitor. CD4+ and CD8+ T cell (D) frequency of total CD45+ T cells and IL-17 production (E and F) as frequency of each parent population in response to IAA treatment with and without AhR inhibitor