Combined effects of two HAT inhibitors on galactose-induced cataracts.

(A) The upper figure shows micrographs taken after incubation without inhibitors, and the lower figure shows micrographs taken after incubation with inhibitors. The number of incubation days is shown in the upper left corner of the photograph. (B) The image on the left (+) shows sections prepared after incubation in galactose-containing medium, followed by addition of CBP30 (10 μM) + CPTH2 (80 μM), and H&E staining. The image on the right (-) shows a section prepared after incubation in galactose-containing medium without inhibitors, followed by H&E staining. (C) Quantification of lens opacity. The opacity of the cortical region of the lens was calculated as previously indicated by subtracting the opacity value measured before addition of the inhibitor from the opacity after addition of the inhibitor [15]. Data are expressed as the mean ± SE. Samples used for quantification are shown in S2 Fig.