Supplemental figure S1 and S2

Supplemental figure S1. Time course of the animal study. Uni-Nx: Unilateral nephrectomy of the right kidney; Poles amputation: Amputation of the poles of the left kidney; BP: Blood pressure; Blood 1 and Blood 2 were taken from retro-orbital venous plexus; Blood 3 was taken from the abdominal aorta. Supplemental figure S2. The differentially expressed genes in kidney detected by RNA-seq transcriptome. (A) Venn diagram of the differentially expressed genes from RNA-seq transcriptome for 5/6Nx + PBO, 5/6Nx + TELM, 5/6Nx + 3mg EMPA and 5/6Nx + 15mg EMPA groups. The circles represent the total number of statistically significant different signals (P value 1) in 5/6 Nx + 3mg EMPA, 5/6 Nx + 15mg EMPA and 5/6 Nx + TELM groups in comparison to 5/6 Nx + PBO in kidney according to RNA-seq transcriptome data. (B, C, D, E) Volcano plot of RNA-seq transcriptome data displaying the pattern of gene expression values for every treatment group relative to 5/6Nx + PBO group. The genes are coloured if they pass the thresholds for p value=0.05 and log2 (fold change) = ±1, orange if they are upregulated and blue if they are downregulated. Significantly differently regulated genes confirmed by qRT-PCR were labelled. (B) 5/6Nx + 3mg EMPA versus 5/6Nx+PBO; (C) 5/6Nx + 15mg EMPA versus 5/6Nx+PBO; (D) 5/6Nx + TELM versus 5/6Nx+PBO; (E) 5/6Nx + PBO versus sham + PBO. Sham: Sham operation; 5/6Nx: 5/6 nephrectomized rat model; PBO: Placebo; TELM: Telmisartan; EMPA: Empagliflozin; qRT-PCR: Quantitative Reverse Transcription Polymerase Chain Reaction.